Lab 01/28/2020

Hello everyone,

in today's lab, I streaked a TGY plate (my second time ever streaking) with D.geo for Chad to propagate it for the experiment we will be conducting next week. More information on the experiment will be coming soon.

Comments

Post a Comment

Popular posts from this blog

Bioinformatics - Assignment #1 (Week 1)

09/09/2020 Anthony Luna Today I finished the first assignment of the bioinformatics project focusing on proteins .  My assigned protein is Q5T084 AMY1B , also known as alpha-amylase 1B polypeptide (Reactome.org). It is found in Homo sapiens and aids in the bodily function of digestion and absorption. Specifically, digestion and absorption of branched starch, amylopectin, and linear starch, amylose (Reactome.org). All of which takes place in the extracellular region of various cells. Moving onto the size, mass, and structure of alpha-amylase 1B. The protein alpha-amylase is folded into three distinct domains, A, B, and C. Yet, my primary focus is on domain B, which has a length of 105 amino acids, aa, and a mass of 12.4 kilo Daltons, kDa (Proteinatlas.org). Alpha-amylase 1B is described as projecting off of domain A in a loop like fashion. However, when a calcium ion binds to domain B, it is then described as relatively rigid (Schutte) . As for diseases involving alpha-amylase 1B, ...
Read more

Lab 09/17/19

Image
In today's lab, Shawn and I made 500mL of TGY (Broth) and TGY (Agar). During our time making the medium, Shawn brought to my attention an imperative step I was missing in my protocol! That was before we add any of the substances to our flask we must first measure and pour half the desired volume in our flask (250mL). Very thankful he brought that to my attention so moving forward I won't have any errors in regards to volume. Another task I performed today was gram staining Deinococcus Radiodurans (D. rad) however this time I wasn't picking my colony from a plate instead I picked it from Broth. The results I received weren't as favorable as I was anticipating due to the fact there were some abnormalities present. D. rad came out gram-positive (purple) instead of gram-negative (pink) which is how D. rad should stain. In addition, I believe possible contamination was present due to spherical gram-positive artifacts or error in my alcohol prep.
Read more

Bioinformatics- Assignment #6

P04745-Alpha-amylase 1 Uniprot ID: AMY1_HUMAN Protein: Alpha-amylase 1 Gene: AMY1A Diseases associated with alpha-amylase: None to be noted. Catalytic activity: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units. Cofactor: Calcium ion (Ca2+) and Chloride ion (Cl-) Alpha-amylase was found to be rather mildly expressed exclusively in the pancreas. No other organs/regions could be noted.  Some aspects of alpha-amylase I am curious about are, one, why is there such little data about it? Two, why would it be expressed only in the pancreas and not somewhere along the digestive tract?  One project idea I have in mind is further investigating where else alpha-amylase could possibly be expressed, and discovering why it is expressed higher in one region than another. 
Read more