Lab 09/17/19

In today's lab, Shawn and I made 500mL of TGY (Broth) and TGY (Agar). During our time making the medium, Shawn brought to my attention an imperative step I was missing in my protocol! That was before we add any of the substances to our flask we must first measure and pour half the desired volume in our flask (250mL). Very thankful he brought that to my attention so moving forward I won't have any errors in regards to volume. Another task I performed today was gram staining Deinococcus Radiodurans (D. rad) however this time I wasn't picking my colony from a plate instead I picked it from Broth. The results I received weren't as favorable as I was anticipating due to the fact there were some abnormalities present. D. rad came out gram-positive (purple) instead of gram-negative (pink) which is how D. rad should stain. In addition, I believe possible contamination was present due to spherical gram-positive artifacts or error in my alcohol prep.


Comments

  1. Monica SanchezSeptember 21, 2019 at 3:11 PM

    It appears you learned a lot during this lab time! It's very nice that you have someone there to help you learn from your mistakes. That's truly the best way to learn. I hope your results are more favorable next time!

    ReplyDelete
    Replies
    1. Anthony LunaSeptember 25, 2019 at 1:55 PM

      I did indeed! It is certainly helpful. Shawn, April and Kristin are very knowledgeable individuals and I'm learning a lot working with them.

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