In today's lab, Trevon and I prepared for a transformation we are going to run on D. rad next Friday and Saturday. However, a more thorough update will come next week when we have completed it.
Today's lab was fairly short. April had me look at D. rad and D. pima under the microscope using a hemocytometer slide. We usually stain the cells but today we did not because April just wanted to take a look at them. However, next week we are going to actually count them once we have conducted our assay's. Below is an image of what a hemocytometer slide looks like. The cells on the slide are D. rad.
Today, I assisted Shawn with his project which has to do with V. fischeri. We made twelve serial dilutions consisting of algae and lysade and then ran a standard curve with a 96 well plate. In hopes to see how V. fischeri luminescence is effected.
In today's lab, I made 50mL of Tris HCl buffer in preparation for the assay we are going to run on Friday. I also made two more 1:1000 dilutions. Other than that today's lab was quite fast.